Estimation of active compounds in Ganoderma spp. and evaluation of its activity against leaf spot disease caused by Alternaria tenuissima on tomato

Abstract

This study aims to evaluat some active compounds of Ganoderma spp. to inhibit the growth of the pathogenic fungus Alternaria tenuissima in the laboratory and control leaves spot disease, inducing plant resistance and encouraging its growth in the field on tomato plants. The results showed the highest inhibition of the pathogenic fungus A. tenuissima growth by the filtrates of the G. applanatum Has.AA-6 , G. lucidum Has.AA-7,  G. resinaceum 1049, and G. resinaceum Has.AA-8 growing in Malt Extract Broth medium, The diameters of the pathogenic fungus colonies were 2.79, 2.95, 3.17, and 3.19 cm, respectively.The highest chitinase and  β-glucanase activities were in the interaction treatment with (extract + filtrate of G. lucidum) (1:1)  in present of  A. tenuissima, reached 2.532 and 1.774   units/ml, respectively in the Agro-Tip tomato variety. The same treatment achieved a high rate of reducing the percentage of infection severity against  A. tenuissima, reaching 15.33, 17.07and 20.36% for the cultivars Agro-tip ,S25 and Rawaa ,respectively. Agro-tip cultivar treated with Extract + filtrate of G. lucidum in present of  A. tenuissima  also achieved the highest plant height, plant dry weight and yield reached to 148.56 cm, 112.38g and 7812.78 g.plant^-1 , respectively.