Effect of plant extracts on inhibiting internal contaminants in vitro

Abstract

These study experiment were carried out in the Plant Tissue Culture Laboratory of the Department of Plant Production Technologies - Postgraduate Studies - Technical College of Mussaib - AL-Furat AL-Awsat Technical University for the period from 19/12/2022 to 4/15/2023.The present experiment aimed to determine the effect of plant extracts (cardamom, cloves, and haramel) at concentrations (0, 2, 4, 6) g. L^-1 of each in MS medium grown with saffron corms in tissue culture, to follow up the effect of extracts on growth results. The results of the triple intervention between the second, third, and fourth weeks with clove extract at a concentration 2 g. L^-1 was signification to other interventions in giving the highest value for the success of Implants remaining free of contamination, amounting to 25.00. There was also no significant effect of the plant extracts on the apparent characteristics of the saffron corm. The clove extract in the nutrient medium supplied with a concentration of (2 g.L-1) outperformed the rest of the plant extracts by giving it the highest average number of shoots, which reached 6.75 shoots, but it was not significant. Also, the prepared nutrient medium (6 g.L-1) of cardamom extract was not significantly superior in giving it the highest average shoot length of 9.50 cm, While the control treatment (0) gave the highest average number of roots reaching 63.8 roots, and the average length was 15.00 mm at a concentration of 4 g. L^-1 for cardamom extract.