A study of the effect of temperature on the activity of the glutaminase enzyme and the kinetic values in calcareous soil

Abstract

The study was carried out in Department of Soil Sciences and Water Resources Lab, College of Agriculture and Forestry, to determine the kinetic parameters (maximum speed Vmax and Substrate matter concentration Km) and to show how temperature influences the activity and performance of the Glutaminase enzyme. therefore, clay loam soil from the Rashidieh district was selected, and it was treated with various Substrate enzyme concentrations (0.15, 0.25, 0.5, 0.75, and 1) molar under various temperature influences (10, 20, 30, 40, 50, 60, and 70) Cᵒ.

The results demonstrated: a definite influence of the substrate on the enzyme's activity, as the enzyme's activity increased as the amount of added substrate got in concentration. The greatest value for the Glutaminase enzyme activity [S]/ V was (0.019) microgram NH4-N g^-1. 2 hr^-1, which came as a result of the substrate's concentration of (1) mmol. The enzyme Glutaminase [S] /V had the lowest activity, measured in micrograms (0.004) NH4-N g^-1. 2h^-1, as a result of the lowest substrate concentration (0.1) mmol. According to the research results, the Vmax value was reached  (0.815) micrograms of NH4-N g^-1 2hr^-1. Km had a value of (0.033) mmole. The Glutaminase enzyme's activity increased as the incubation temperature was raised when the substrate concentration was (0.5) molar. The highest value for Glutaminase enzyme activity was (213.70) micrograms of (NH4-N g^-1 2hr^-1), which was obtained from incubating the soil at the highest temperature (60) Cᵒ, as the Glutamines enzyme activity values increased.