Detection of the Effect of a Natural Product Syzygium aromaticum on Bacteria Causing Urinary Tract Infections Isolated from Patients at Tikrit Teaching Hospital

• Maream Kaream Khalaf Al-Hallawi1* , Firas Adnan Hussein2

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  108 samples were collected from patients suffering from urinary tract infections at Tikrit Teaching Hospital. Bacterial growth testing was performed on the studied samples. The bacterial species were isolated and identified, activated on special culture media, and treated with aqueous and alcoholic extracts of clove. The extracts were prepared, and their active components were analyzed using GC-MS. Three concentrations of each extract were prepared and tested against the bacterial isolates to determine the inhibition diameters. The data were collected and statistically analyzed. The results of the bacterial diagnosis showed that bacterial pathogens were the most prominent factor in the infections, with Escherichia coli and Klebsiella  species  bacteria recording the highest prevalence rates among the isolated groups. Treatment of the isolated species with aqueous and alcoholic clove extract proved the efficacy of the alcoholic extract, which showed a clear advantage in destroying bacterial cells. The inhibition diameters peaked at a concentration of 750 mg/ml, reaching 30 mm against Escherichia coli and 28 mm against Enterococcus faecalis. The efficacy of the aqueous extract: The aqueous extract achieved a maximum inhibition diameter of 22 mm against Enterococcus faecalis at a concentration of 750 mg/ml. Clove extracts (both aqueous and alcoholic) exhibited clear inhibitory effects against the bacteria causing these infections. The alcoholic extract contains eugenol (86.25%) supported by caryophyllene (11.20%), giving it a high efficacy. The aqueous extract contains long-chain carbonic acids (diisooctyl phthalate and docosanoic acid) exceeding 66%.

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