Manufacture of probiotic dairy beverage using Buffalo milk's concentrated whey proteins and dried grape juice powder

• Teba Saleem Alshmary1* Zahraa Ressan Karem1 Dhiaa Ibraheem Albadrany1

  Author

Given the recognized health benefits and high nutritional value of dairy products, this study aimed to develop a fermented dairy beverage fortified with a probiotic strain (Lactobacillus plantarum) using buffalo milk whey protein concentrate at varying concentrations, in combination with dried grape juice and sucrose. A control treatment (C) was prepared using buffalo milk protein concentrate only. Three treatments (G1, G2, G3) received protein concentrate at 1.0%, 2.0%, and 3.0%, respectively, without the addition of the probiotic. Three additional treatments (G4, G5, G6) received the same protein concentrate levels (1.0%, 2.0%, and 3.0%) with the probiotic added at a concentration of 10⁸ CFU/mL. Chemical analyses included the determination of moisture, protein, fat, ash, carbohydrate, and titratable acidity. Physical assessments comprised viscosity, density, and pH, in addition to microbiological and sensory evaluations.

The results showed a marked decrease in moisture content with increasing levels of whey protein concentrate addition. In contrast, the percentages of protein, fat, carbohydrates, and ash increased in the treatments with added juice compared to the control treatment.

Titratable acidity increased, and pH decreased significantly in the treatments fortified with the probiotic during storage periods of 1, 7, 14, and 21 days.

Physical tests revealed that viscosity and density values increased in all treatments in direct proportion to the increasing levels of whey protein addition, compared to the control treatment, with the probiotic-containing treatments showing superior results.

Microbiological examinations indicated no contamination with E. coli, Staphylococcus aureus, yeasts, or molds in any of the treatments.

Furthermore, sensory properties were improved in the probiotic-fortified treatments compared to the control treatment.

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