Effect Using Mushroom Waste Substitute With Wheat Bran To Aged Laying Hens Diet On Histological And Physiological Performance

• Suhaib Ahmed Khalaf 1, Baraa Hameed Mousa2 , Idham Ali Abed

  Author

This study was conducted at animal production farm belong to College of Agriculture, University of Anbar, from 7^th April to 21^st July , 2025. The study aimed to investigate using of oyster mushroom culture products grown on crushed Reed Plant (  officinarum) as a substitute with wheat bran in diets of aged laying hens and its effect on productive performance. The experiment lasted for four periods (28 days/period). One hundred - eight of Lohmann Brown (50-weeks old) were used in study were divided into six experimental groups, with three replicates (six hens per replicate). The experimental treatments were as follows: T1: standard diet without any additives. T2: standard diet with 20% wheat bran replaced by mushroom culture waste ; T3: standard diet with 40% wheat bran replaced by mushroom culture waste ; T4: standard diet with 60% wheat bran replaced by mushroom culture waste ; T5: standard diet with 80% wheat bran replaced by mushroom culture waste ; T6: standard diet with 100% wheat bran replaced by mushroom culture Waste were cultivated by inoculating mycelium on wheat seeds. Reed Plant (Saccharum officinarum) straw (80%) and wheat straw (20%) were used. After drying and grinding, the inoculated bags were placed in an incubation chamber until the mushrooms reached maturity. A significant decrease (P≤0.05) was observed in blood Glucose concentration, AST, LDL, Cholesterol, and PCV in treatment T6, which used 100% mushroom residue powder, compared to the control treatment T1. A significant increase (P ≤ 0.05) was observed in total plasma protein, albumin, and RBC concentrations in treatment T6. A significant increase (P ≤ 0.05) was also observed in hemoglobin levels in treatment T4. White blood cell counts showed a significant increase (P≤0.05) in treatment T2. Histological measurements showed significant increases in villus thickness, villus diameter, and villus depth in both treatments T6 and T5. It can be concluded that birds fed mushroom residue exhibit greater antioxidant resistance, higher protein levels, and enhanced immunity.

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