The synergistic effects of dietary fibers and probiotics on gut bacteria, microbial balance, and inflammatory markers against castor oil–induced diarrhea in mice

• Adnan Nsaif Hammadi Al-Qaraghli1 , Hani Sabbar Ayed2

  Author

     This study was conducted in the postgraduate laboratories of the College of Agriculture, University of Baghdad, and in the animal house of the College of Veterinary Medicine, University of Tikrit, to determine the synergistic effect of dietary fiber and probiotics on intestinal bacteria, microbial balance, and inflammatory markers in mice with diarrhea treated with castor oil.The study results showed that administering castor oil to albino mice led to a significant increase (p≤0.05) in the levels of the inflammatory markers TNF-α and IL-6, and also caused a significant increase in the number of intestinal bacteria, while the number of lactic acid bacteria decreased.The results indicated positive effects represented by a decrease in the level of inflammatory factors and an increase in the total number of lactic acid bacteria in the intestines when oral administration of the probiotic Lb. acidophilus alone or with feeding with carrot powder or brown rice or both, and the total number of colonic bacteria decreased as a result of the increase in the number of lactic acid bacteria.We conclude that oral administration of the probiotic and feeding with dietary fiber powder improved parameters of inflammatory factors and microbial balance in male rats with diarrhea.

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