Effect of Mannan Extracted from Saccharomyces boulardii on Hematological and ‎Immune Parameters in Rats with High-Fat Diet–Induced Metabolic Stress

• Wael Mahdi Salih1, Athraa Harjan Mohsen2

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A high-fat diet is a major contributor to metabolic and inflammatory disorders that negatively affect ‎hematological and immune parameters. This study aimed to evaluate the physiological effects of Pre-extracted and purified mannan ‎extracted from the cell wall of Saccharomyces boulardii in rats subjected to high-fat diet–induced ‎metabolic stress . Thirty-two male albino rats (Rattus norvegicus) were randomly assigned into eight groups (n= 4 per group ) ‎control (G1),  high-fat diet (G2),  mannan-treated groups at doses of 25, 50, 75, and 100 mg/kg (G3 to G6), ‎atorvastatin-treated group (G7), and a standard diet with mannan (G8). Hematological parameters ‎including RBC, HGB, PCV, MCV, MCH, WBC, lymphocytes (LYM), and platelets (PLT) were assessed‏.‏ The high-fat diet (G2) exhibited significant alterations, including increased WBC (10.86 ×10³ cells/mL) and ‎PLT (395.8 ×10³ platelets/mL) alongside decreased RBC (7.793 ×10⁶ cells/mL)  HGB (9.79 g/dL)  PCV ‎‎(38.52%)  and MCH (12.50 pg) compared with the control (G1), which showed normal values, RBC (8.55 ‎‎×10⁶ cells/mL); WBC (7.81 ×10³ cells/mL); LYM (8.00 ×10³ cells/mL); HGB (14.50 g/dL); PCV (41.12%); ‎MCH (16.98 pg); MCV (48.27 fL); PLT (374.2 ×10³ platelets/mL)  LYM also decreased in (G2) to (7.04 ‎‎×10³ cells/mL) while MCV slightly increased (49.55 fL) .‎ Mannan treatment demonstrated dose-dependent improvement (G3) showed limited effects whereas (G4) ‎showed significant  improvement , The best response was observed in (G5), with increased RBC (9.41 ×10⁶ ‎cells/mL), LYM (9.065 ×10³ cells/mL), and HGB (14.91 g/dL), along with reduced PLT (336.2 ×10³ ‎platelets/mL) and near-normal MCV (48.35 fL). G6 showed moderate improvement, while atorvastatin ‎‎(G7) produced partial recovery. (G8) remained within physiological limits‏.‏ These findings indicate that mannan exerts significant regulatory effects on hematological parameters, with ‎optimal efficacy at moderate doses, supporting its potential as a functional dietary supplement in managing ‎metabolic disorders.‎

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