Biochemical blood parameters and oxidative indices of broiler blood affected by locally extracted aloe vera oil in different ways

• Mustafa Lateef Abd Al-Hamadani1 Jassim Kassim Al-Gharawi2 and Jinan Abed Abbas Al-Shibawi3

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The study consisted of two parts: the first was conducted at the Al-Baz hatchery in the holy city of Karbala on October 30, 2025 (18 day of incubation). The second part was conducted at the poultry farm, Agricultural Research and Experiment Station, College of Agriculture, Al-Muthanna University, from November 2, 2025, to December 7, 2025, for a period of 35 days. Two hundred and seventy one-day-old, unsexed Ross 308 broiler chicks, 40 gm weight each, were used in the study. The chicks were raised in a 40×10 m hall. They were housed in four-tiered batteries, each tier containing a 1×1.5 m cage. The chicks were randomly distributed into six experimental treatments, with 45 chicks per treatment. Each treatment had three replicates (15 chicks per replicate). The experimental treatments were as follows: T1: Negative control (no injection). T2: Positive control (In ovo injection of hatching eggs with 0.01 ml of sterile distilled water). T3: In ovo injection of hatching eggs with 0.01 ml of locally extracted aloe vera oil. T4: Oral administration of 0.25 ml/chick of locally extracted aloe vera oil to seven-day-old chicks. T5: Add of 2 ml/liter of locally extracted aloe vera oil to drinking water. T6: Add of 4 ml/kg of feed to locally extracted aloe vera oil. The results showed that a significant decrease (P≤0.05) on some blood biochemical parameters, namely cholesterol, triglycerides, and glucose concentrations. Conversely, a significant increase (P≤0.05) on albumin, globulin, and total protein concentrations in all treatments administered locally extracted aloe vera oil compared to the control treatments. The best results were obtained in treatment T5. Regarding oxidation, a significant decrease (P≤0.05) on the concentration of malondialdehyde. Conversely, a significant increase (P≤0.05) on the concentrations of both glutathione peroxidase and catalase enzyme in all treatments administered locally extracted aloe vera oil compared to the two control treatments.

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